Figure 1.

Characterization of antibodies against opioid receptors. (A) Rat striatal extracts (10 μg of protein) treated with Endo-β-N-acetyl-glucosaminidase F1 (100 milliunits/ml) (see Materials and Methods) were subjected to trans-immunoblots using the antibodies against MOR, DOR, and KOR. Arrows indicate immunoreactive protein bands. MW, molecular weight; PS, protein staining. (B–D) Single-label TSA immunostaining of the upper cervical spinal cord by using the antibodies against MOR (B), DOR (C), or KOR (D). Arrows indicate the superficial layers of the dorsal horn of the spinal cord. (E) No specific immunoreactivity was found in the spinal cord processed for the TSA protocols in the absence of the primary antibodies. Scale bar = 500 μm.