Figure 2.

Co-injected SKOV3.ip1-GFP and RFP cells yield chimeric tumors. Equal numbers of SKOV3.ip1-GFP and SKOV3.ip1-RFP cells were injected as a single-cell suspension into the peritoneum of nude mice. (A–C) Large tumors on the omentum are both GFP-positive (GFP-filter) (A) and RFP-positive (RFP-filter) (B). White boxes: magnified region shown in (C). (C) A 5X magnified composite image of the tumor from (A,B) showing a mixture of GFP- and RFP-positive cells. (D,E) Chimeric tumors on the mesentery have patches of green and red fluorescence. A clonal tumor that is only GFP-positive can be seen (E), (arrow). (F) Endpoint of a mathematical simulation initialized with a mixed GFP/RFP spheroid of 56 cells attached to the mesothelial surface of the intestine. A 180 × 180 × 180 μm lattice (5.832 mm³) is partitioned into layers of smooth muscle (brown), extracellular matrix fibers (teal), mesothelium (dark blue), and vessel (red) creating a 0.84 mm³ tissue layer. Above the tissue is peritoneal fluid. The 3-D image shows a chimeric tumor (orange and green cells) after 7 days of growth.