Table 3.

Exploration of adhesion parameters in spheroids attached to the surface of the small intestine.

Parameters tested			Results	Spheroid images
				2-D (tumor center)	3-D
Low homotypic adhesion and low heterotypic adhesion to non-tumor cells			By 24 h, tumor cells show reduced volume and the spheroid is no longer cohesive
Cell type 1	Cell type 2	Adhesion 1 ⇔ 2
PTC	PTC	20
PTC	other	20
other = PF, ECM, VM, and SM
Low homotypic adhesion and high heterotypic adhesion			By 24 h, the spheroid has fragmented while the mesothelium has aggregated
Cell type 1	Cell type 2	Adhesion 1 ⇔ 2
PTC	PTC	20
PTC	other	Tested 0, 1, 3, and 5
High homotypic adhesion and high heterotypic adhesion			By 12 h, the spheroid has begun to disintegrate and the mesothelium has aggregated at the base of the spheroid
Cell type 1	Cell type 2	Adhesion 1 ⇔ 2
PTC	PTC	0
PTC	other	0
High homotypic adhesion and low heterotypic adhesion No surface area constraint			At 24 h, a coherent spheroid with a similar appearance to the SKOV3.ip1 in vivo tumors can be seen. The mesothelium remains intact. However, cells are abnormally convoluted
Cell type 1	Cell type 2	Adhesion 1 ⇔ 2
PTC	PTC	0
PTC	other	Tested 10 and 20
High homotypic adhesion and low heterotypic adhesion Additional surface area constraint			This model most closely approximates the shape of SKOV3.ip1 spheroids and cells in vivo as shown at 12 h
Cell type 1	Cell type 2	Adhesion 1 ⇔ 2
PTC	PTC	0
PTC	other	See Table 2

Abbreviations are the same as in Table 2.