Figure 3.

Absence of tolerance to the amnesic-like effect and tolerance to the hypothermic effects during chronic THC treatment. DI values for 12 consecutive tests on mice (n=30–35 mice/group) treated chronically (black arrows for administration days) with THC 10 mg/kg, 6 days, once daily) (black square), or vehicle (white triangle) in the object-recognition memory test (a). Effects of THC (10 mg/kg) or vehicle (n=10–15 mice/group) on body temperature during the 6 days of treatment (b). Quantitative analysis of p70S6K(T389) immunodetection in the hippocampus of mice (n=4–5 mice/group) treated acutely or chronically with THC or vehicle, and sacrificed 30 min after test 5, or immediately after test 8 and test 12 of the experimental protocol (c). Data are expressed as mean±SEM. *P<0.05, **P<0.01, ***P<0.001 compared with vehicle treatment.