. Author manuscript; available in PMC: 2013 May 17.

Published in final edited form as: J Proteome Res. 2011 Dec 27;11(2):808–817. doi: 10.1021/pr2006704

Table 1.

Isoelectric focusing and mass spectrometric properties of proteotypic peptides for prostate specific antigen (PSA).

Protein	MW (kDa)	Proteotypic Peptides^a	ESP Value^c	pI	dPC	MH+ (mono)	Charge state, x, (Q1)	Precursor ion (m/z), Q1	Product ions (m/z), Q3	Collision Energy (V)	Quantitation transition (m/z)
Prostate specific antigen	29	LSEPAELTDAVK	0.84	3.9	3.0–5.0	1272.7	2	636.8	775.4, 846.5, 943.5	26, 22, 14	636.8 → 943.5
		LSEPAEL[¹³C₆]TDAVK				1278.7	2	640.8	781.4, 852.5, 949.5	26, 22, 14	640.8 → 949.5
		IVGGWEC_amcEK^b	0.36	4.3	3.0–5.0	1078.5	2	539.7	809.3, 866.3, 965.4	15, 15, 15	539.7 → 866.3
		IV[¹³C₆]GGWEC_amcEK^b				1083.5	2	542.2	809.3, 866.3, 971.4	15, 15, 15	542.2 → 866.3

The stable isotope labeled amino acids are indicated in bold.

Carbamidomethyl group were introduced during peptide synthesis.

ESP value – probability value.