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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Autoimmun. 2012 Jul 28;39(4):398–411. doi: 10.1016/j.jaut.2012.06.005

Fig. 3.

Fig. 3

Competitive binding to dsDNA among the different PL9–11 mAbs. Serial dilutions of PL9–11 mAbs (“competing antibodies”) were bound to dsDNA coated ELISA plates, followed by each individual isotype (“test antibody”) at a fixed concentration of 0.5 μg/ml to assess the relative affinities of the test and competing antibodies to dsDNA. The title of each panel denotes the test antibody against which each of the other isotypes is competing for binding in that particular experiment. The amount of the test antibody that remained bound to dsDNA was detected with an isotype-specific secondary antibody, as shown in the illustration. Binding of the test antibody to dsDNA without the presence of a competitor was defined as 100%. Each dilution of the competing antibody was done in triplicate, and the mean and SD values are presented on the graph. Data are representative of three experiments performed in duplicate.