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. Author manuscript; available in PMC: 2013 May 17.
Published in final edited form as: J Comp Neurol. 2010 Mar 15;518(6):800–814. doi: 10.1002/cne.22243

Figure 2.

Figure 2

Rod photoreceptor cell loss and regeneration following metronidazole treatment of Tg(zop:nfsB-EGFP)nt19 zebrafish. Adult fish were treated with 10 mM metronidazole for 24 hours. Following treatment, a minimum of five fish were sacrificed (24h Met., B); the remaining fish were transferred to a recovery tank and collected following 24, 48, 72, 96, and 168 hours and 28 days of recovery (C–H, respectively). A: Control Tg(zop:nfsB-EGFP)nt19 fish were not treated with metronidazole. B: After 24 hours of metronidazole treatment, rod photoreceptors throughout the retina appeared disorganized. C: After 24 hours of recovery, there was an obvious reduction in the number of EGFP-expressing dorsal and ventral ONL cells, consistent with rod photoreceptor death. D–F: After 48 hours of recovery, a maximal amount of rod cell loss was achieved in the ventral retina (D), based on the absence of EGFP expression, whereas the dorsal retina retained some EGFP-expressing rods through 72 hours of recovery (E). Regenerated ventral rods were first observed following 72 hours of recovery (E) and regenerated dorsal rods were found following 96 and 168 hours of recovery (F,G). H: By 28 days of recovery, rod photoreceptors had fully regenerated throughout the retina. ONL, outer nuclear layer. Scale bar = 150 μm in A (applies to A–H). ONL, outer nuclear layer.