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. Author manuscript; available in PMC: 2013 May 17.
Published in final edited form as: J Comp Neurol. 2010 Mar 15;518(6):800–814. doi: 10.1002/cne.22243

Figure 9.

Figure 9

Metronidazole treatment results in the loss of only the subset of rods expressing the nfsB-EGFP transgene. Tg(zop:nfsB- EGFP)nt20 zebrafish were treated with 10 mM metronidazole for 24 hours and transferred to a recovery tank for 24, 48, 72, 96, 168 hours, and 28 days of recovery. Cell death was assessed by the loss of EGFP fluorescence, whereas rhodopsin immunolocalization was used to examine the non-transgenic rods. A: The control retina showed both EGFP-expressing rods in the ONL and rhodopsin expression in the ROS. B–D: The number of EGFP-expressing rods decreased from 24 hours of metronidazole treatment through 48 hours of recovery, whereas significant rhodopsin expression persisted in a thick layer of ROS due to the preservation of rods not expressing the nfsB-EGFP transgene. E: By 72 hours of recovery, newly regenerated EGFP-positive rods were observed (arrow). F–H: The number of regenerated EGFP-expressing rods increased through 96 (F) and 168 (G) hours of recovery until the retina, at 28 days of recovery (H), was indistinguishable from the control (A). ONL, outer nuclear layer; ROS, rod outer segments. Scale bar = 20 μm in A (applies to A–H).