Fig. 1.

Uric acid increases intracellular oxidative stress in cells incubated with UA 714 μM (12 mg/dL) for 48 h. This effect was prevented by cotreatment with apocynin suggesting the participation of NADPH oxidase in this effect (A) (n=3, triplicates). Uric acid reduces NO bioavailability in HAEC incubated with UA 714 μM (12 mg/dL) for 48 h as suggested by a significant reduction in nitrates/nitrites measured by DAF2-DA fluorescence in fixed cells (B) (n=3, triplicates) and as secreted nitrates/nitrites in the cell culture media (C) (n=2, triplicates).