Fig. 2.

Uric acid reduces mitochondrial mass in HAEC incubated with UA 714 μM (12 mg/dL) as showed by a significant decrement of mitDNA at 24 and 48 h (A) (n=3, triplicates). This effect was in association with a lower fluorescence intensity using the specific mitochondrial dye Mitotracker Orange^™ (B) at 48 h of incubation with UA (n=2, triplicates). Uric acid also alters the expression and bioactivity of mitochondrial enzymes and reduces ATP levels in HAEC incubated with UA 714 μM (12 mg/dL) for 48 h. Mitochondrial ECoAH-1 expression (C) and ACO-2 activity (D) were significantly reduced in UA treated cells (n=3, triplicates). UA incubation for 48 h also reduced basal intracellular ATP concentration (E) (n=3, triplicates).