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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cell Signal. 2013 Feb 13;25(5):1136–1148. doi: 10.1016/j.cellsig.2013.01.026

Figure 4. rPMT induces CTGF mRNA in quiescent 3T3 cells.

Figure 4

Serum-starved 3T3 cells were treated with 50 ng/ml of rPMT for 24h. Total RNA was prepared from control and rPMT treated cells, reverse transcribed, and applied to Affymetrix arrays. (A) shows cluster of genes that are altered due to rPMT treatment. Experiments were performed in quadruplicates, and average induction values were obtained. Data analysis showed that 1774 probe sets representing 1185 genes were up-regulated and 1599 probe set representing 1110 genes were decreased by at least 2-fold (treated vs. non-treated). CTGF, a CCN family member, is the most upregulated gene following rPMT treatment (Table 1). RNA was isolated from both control and cells exposed to either a fixed concentration of rPMT for indicated period of times (B) or to different concentrations of rPMT for 24h (C) and analyzed by RT-PCR using CTGF primers.