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. 2013 May 21;4(3):e00321-13. doi: 10.1128/mBio.00321-13

FIG 2 .

FIG 2 

Expression of 3× myc-tagged VSPs on the membrane surface of Giardia trophozoites. (A) Detection of the expression of N-myc-tagged VSP9B10A on a Western blot using myc-Ab. Most of the tagged VSP was present in the organic phase. (B) Immunofluorescence assay of the nonpermeabilized cells with double staining of N-myc-VSP9B10A using myc-Ab (FITC) and mAb 9B10 (Cy5). No cell surface expression of VSP was detected. (C) Detection of the expression of C-myc-tagged VSP9B10A on a Western blot using myc-Ab. Most of the myc-VSP9B10A was present in the organic phase, too. (D) Immunofluorescence assay of the nonpermeabilized cells with double staining of C-myc-VSP9B10A using myc-Ab (FITC) and mAb 9B10 (Cy5). The cell surface was stained by both antibodies and demonstrated the same pattern of staining.