FIG 5 .

Motifs 1 and 2 may be involved in localizing VSP to Giardia trophozoite membrane. (A) Sequence alignment of the C-terminal portions of all the 11 VSP proteins shown to localize to the cell membrane surface in Fig. 3 and 4. (B) Sequence alignment of the C-terminal portions of all the 10 non-VSP proteins shown to confine to the cytoplasm in Fig. 3 and 4. Motif 1 is a stretch of 45 amino acids, whereas motif 2 is at the C terminus with a length of 38 residues. The conserved regions in motifs 1 and 2 were highlighted. None of the non-VSPs has the conserved regions in motif 1, but the non-VSPs 201, 259, 213, and 333 have the conserved motif 2.