FIG 7 .

Sequence alignment of the C-terminal portions among the 73 newly identified VSPs in Giardia. Motif 1 is shown in the red box. Motif 2 is shown in the green box. The conserved sequences were highlighted in both motifs. The gene identifier from GiardiaDB and VSP designations for each VSP are listed. The VSPs experimentally tested and confirmed in this study are in red. The three subgroups labeled I, II, and III are derived from a distance tree analysis among the 73 C-terminal sequences using Clustal W (see Fig. S3 in the supplemental material).