Figure 2.

In vivo assay for DC generation from C/EBPα-targeted progenitors. (A) FACS analysis of CD45.2⁺ donor–derived cells, from 5000 transplanted KSL of C/EBPαfl/fl or Mx1^–Cre⁺ C/EBPαfl/fl–transplanted mice, for CDP (Lin^–, Flt3⁺, FcγRII/III^–, cKit⁺, CD115⁺, CD11c^–) from bone marrow, or cDC (CD19^–, CD3^–, NK^–, CD11c⁺, MHCII⁺) and pDC (CD19^–, CD3^–, NK^–, CD11c⁺, B220⁺) from the spleen show contribution of donor cells to each population. The data shown are representative dot plots from 3 mice per group for 3 independent experiments with similar results. (B) Absolute numbers of total CD45.2⁺ CD19^–, CD3^–, NK^– CD11c⁺ DCs were calculated from total splenocytes (left). B220⁺ subset of cells was determined to be pDC (black), and B220^– MHCII+ cells were cDC (white) and plotted accordingly. CMPs were sorted from either MxCre^– C/EBPαfl/fl or MxCre+ C/EBPαfl/fl mice and analyzed for expression of CX₃CR1 by RT-PCR. The percentage of CDP from total CD45.2⁺ bone marrow are presented from KSL of C/EBPαfl/fl or MxCre⁺ C/EBPαfl/fl–transplanted mice (right). The numbers represent mean ± SD from 4 similarly transplanted mice. One of 3 experiments giving similar results is presented.