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. 2013 Mar 29;121(20):4175–4183. doi: 10.1182/blood-2012-11-466938

Figure 2.

Figure 2

Elevated levels of ROS in the most primitive LSCs from CML-CP–like mice. BMCs were obtained from healthy (black bars) and CML-CP–like (gray bars) mice. (A) ROS were detected with DCFDA in HSCs/LSCs (Lin−c-Kit+Sca-1+) and HPCs/LPCs (Lin−c-Kit+Sca-1−) from 7 healthy (black bars) and 4 CML-CP–like (gray bars) animals. *P ≤ .001 in comparison with HSCs; and P < .05 in comparison with LPCs. (B) ROS were detected with DCFDA in BM-derived subpopulations of Lin−c-Kit+Sca-1+ cells from 2 healthy and 2 CML-CP–like mice: CD34−Flt3− LT, CD34+Flt3− ST, and CD34+Flt3+ MPPs. *P < .05 in comparison with healthy counterparts. (C) ROS were measured with DHE (left panel) and CC-1 (right panel) in Lin−c-Kit+Sca-1+CFSEmax quiescent cells from 7 and 4 normal, and 3 and 3 CML-CP–like mice, respectively. *P < .05 and **P < .005 in comparison with healthy counterparts.