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. Author manuscript; available in PMC: 2013 May 17.
Published in final edited form as: J Immunol. 2011 Sep 28;187(9):4501–4508. doi: 10.4049/jimmunol.1101307

FIGURE 3.

FIGURE 3

DHX9 requires DSRM domain to bind poly I:C. A, Immunoblot using anti-HA Ab of pulldown assays in which purified HA-DHX9 or HA-DHX36 recombinant proteins were incubated with biotinylated poly I:C, poly dA-dT, poly dG-dC, poly dI-dC, CpG A, or CpG B, followed by addition of NA beads. B, Schematic representations of full-length and serial truncations of DHX9. HA-DHX9, DHX9 full size; HA-9ΔDSRM, DHX9-deleted DSRM; HA-9ΔDEXDc, DHX9-deleted DSRM and DEXDc; HA-9ΔHelicC, DHX9-deleted DSRM, DEADc and HelicC; HA-9CΔDUF, DHX9-deleted DUF; HA-9CΔHD, DHX9-deleted HA2 and DUF. Numbers denote amino acid residues. C, Immunoblot using anti-HA Ab of pulldown assays in which purified HA-tagged serial truncations of DHX9 were individually incubated with biotinylated poly I:C, followed by addition of NA beads. D, Immunoblot using anti-HA Ab of pulldown competition assays in which 0.5, 5, or 50 µg/ml CpG B, poly A, poly A:U, poly I:C, or CpG A was added to a mixture of HA-DHX9 protein plus biotinylated poly I:C, followed by addition of NA beads. DEAD, Asp-Glu-Ala-Asp box motif.