Figure 3. PCASIN1-deficient pDCs display severely impaired IFN-α production.

(A) pDCs isolated from WT and PACSIN1-deficient mice were stimulated with 1 µM of CpG-A and CpG-B for 24 h. Cytokine production (IFN-α, TNF-α, and IL-6) was measured by ELISA. The data represent cytokine production by pDCs from individual mice (circle, n = 4/genotype). (B) Isolated pDCs were stimulated with Flu, VSV, and HSV-1 for 24 h. IFN-α and TNF-α production was measured by ELISA. The data represent pDCs from individual mice (circle, n = 3/genotype). A representative result of two independent experiments is shown. (C) WT and PACSIN1-deficient mice were injected intravenously via tail vein with 1 × 10⁷ pfu HSV-1 virus. Sera were collected 6 h and 12 h after infection. Induction of IFN-α (left panel) and IL-6 (right panel) in the sera were measured by ELISA. Sera before virus infection were used as baseline control. Each circle represents one mouse (n = 6 per group). Statistical analysis was performed using an unpaired two-tailed Student’s t-test.