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. Author manuscript; available in PMC: 2013 Aug 11.
Published in final edited form as: Dev Cell. 2013 Jan 31;24(3):296–309. doi: 10.1016/j.devcel.2012.12.003

Figure 2. Expression of wnt11r, wnt4a, and fzd8a during pouch formation.

Figure 2

(A-F) Colorimetric in situs show expression of wnt11r in discrete domains of mesoderm (arrowheads in A and B), wnt4a in ectodermal patches (arrowheads in C and D), and fzd8a in pouch-forming endoderm (E and F).

(G-J) Double fluorescent in situs show co-localization of wnt11r but not wnt4a with nkx2.5 in lateral views (G and I) and higher magnification orthogonal sections (H and J, taken at level of white lines in G and I). Arrowheads indicate mesoderm and arrows ectoderm.

(K) Fluorescent in situ shows co-localization of fzd8a (green) with her5-positive pouch endoderm labeled by GFP immunohistochemistry (red).

(L) Schematic showing expression of wnt11r in mesoderm (blue), wnt4a in ectoderm (yellow), and fzd8a in endoderm (red) during pouch formation.

(M-P) Compared to un-injected siblings (M and O), injection of 5 nl of 5 mM Mtz into nkx2.5:Gal4VP16; UAS:CFP-NTR embryos results in reduced numbers of mesodermal cells expressing nkx2.5 and wnt11r (arrowheads), as well as reduced ectodermal wnt4a expression. Scale bars = 20 μM.