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. Author manuscript; available in PMC: 2013 Aug 11.
Published in final edited form as: Dev Cell. 2013 Jan 31;24(3):296–309. doi: 10.1016/j.devcel.2012.12.003

Figure 3. Roles of Wnt signaling components in pouch and CB cartilage development.

Figure 3

(A-L) Alcama immunohistochemistry (green) shows defects in pouches in mutant, fzd8a-MO, and transgenic embryos. Identifiable pouches are numbered. UAS transgenic embryos (capitalized) were doubly positive for nkx2.3:Gal4VP16. Scale bar = 40 μM.

(M-X) Whole mount views of dissected facial cartilages. Arrows indicated fused or abnormal CB cartilages. Arrowhead indicates abnormal hyoid cartilage in fzd8a-MO embryos.

(Y-AA) Superimposition of initial (blue) and final (red) still images from time-lapse recordings of fifth pouch development in wild-type, wnt11r-/-, and wnt4a-/- embryos. Colored lines indicate cell tracks (shown also below merged images), with filled circles denoting final positions.

(AB) Average pouch cell speed in wild types and mutants. Data represent mean ± SEM and p-values are shown for each comparison.

See also Figures S1 and S2.