Table 1.
Summary of pouch, CB cartilage, and Alcama localization defects.
| Genotype | nPouches | Pouches | Loss of ≥2 Pouches | Loss of 1 Pouch | Abnormal, No Loss | Normal | p-value | nCBs | CBs | nAlcama | Alcama Localization |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Wild type | 100 | 5.0 | 0% | 0% | 0% | 100% | 100 | 5.0 | 55 | 0.07 | |
|
| |||||||||||
| wnt11r- | 222 | 4.3 (4-5) | 0% | 46% | 19% | 35% | 6.61E-40a | 131 | 4.6 (4-5) | 58 | 0.17 |
| wnt4a- | 352 | 5.0 (5) | 0% | 0% | 23% | 77% | 5.71E-08b | 144 | 4.1 (3-5) | 51 | 0.53 |
| wnt11r-; wnt4a- | 45 | 3.4 (2-4) | 20% | 47% | 0% | 33% | 5.97E-12a | 16 | 3.7 (2-4) | 59 | 0.69 |
|
| |||||||||||
| wnt4a-MO | 98 | 4.6 (4-5) | 0% | 20% | 26% | 54% | 2.12E-07a | 74 | 3.8 (3-5) | 61 | 0.52 |
| wnt11r-; wnt4a-MO | 56 | 3.0 (2-4) | 50% | 25% | 0% | 25% | 2.82E-13c | 48 | 3.4 (1-5) | 51 | 0.71 |
|
| |||||||||||
| fzd8a-MO (200uM) | 260 | 3.9 (2-5) | 18% | 26% | 24% | 32% | 1.42E-29a | 253 | 3.8 (2-5) | 58 | 0.69 |
| fzd8a-MO (100uM) | 86 | 5.0 (5) | 0% | 0% | 0% | 100% | 78 | 5.0 | n/a | n/a | |
| fzd8a-MO-II | 212 | 3.9 (2-5) | 19% | 32% | 19% | 30% | 1.87E-32a | 87 | 3.9 (2-5) | 53 | 0.66 |
|
| |||||||||||
| wnt11r-; fzd8a-MO (200uM) | 62 | 3.9 (2-5) | 19% | 35% | 23% | 23% | 0.4977d | 68 | 3.9 (2-5) | 64 | 0.73 |
| wnt11r-; fzd8a-MO (100uM) | 44 | 4.0 (2-5) | 7% | 55% | 7% | 31% | 0.0238c | 38 | 4.1 (3-5) | 55 | 0.64 |
|
| |||||||||||
| wnt4a-; fzd8a-MO (200uM) | 61 | 4.0 (2-5) | 18% | 35% | 21% | 26% | 0.3328d | 65 | 3.8 (2-5) | 64 | 0.78 |
| wnt4a-; fzd8a-MO (100uM) | 42 | 4.0 (3-5) | 17% | 29% | 14% | 40% | 1.29E-07e | 40 | 4.0 (2-5) | 51 | 0.67 |
|
| |||||||||||
| wnt11r-; wnt4a-; | 36 | 2.9 (2-4) | 86% | 6% | 0% | 8% | 0.0185f | 28 | 3.3 (2-5) | 57 | 0.86 |
| fzd8a-MO (200uM) | |||||||||||
|
| |||||||||||
| nkx2.3:Gal4VP16el93 | 78 | 5.0 | 0% | 0% | 0% | 100% | 75 | 5.0 | 52 | 0.09 | |
|
| |||||||||||
| UAS:Wnt11rel421 | 32 | 3.2 (2-4) | 50% | 38% | 0% | 12% | 3.19E-13g | 33 | 3.5 (3-4) | 51 | 0.24 |
| UAS:Wnt4ael48 | 114 | 3.4 (3-4) | 42% | 39% | 0% | 19% | 1.05E-24g | 70 | 3.7 (3-4) | 66 | 0.06 |
|
| |||||||||||
| UAS:DvlΔDEPel190 | 122 | 2.8 (2-4) | 74% | 15% | 0% | 11% | 9.72E-30g | 68 | 3.4 (2-4) | 62 | 0.84 |
| UAS:DN-Rac1el262 | 152 | 3.4 (3-4) | 41% | 27% | 0% | 32% | 4.34E-29g | 77 | 3.8 (3-4) | 68 | 0.63 |
| UAS:DN-Cdc42el268 | 124 | 3.9 (3-5) | 11% | 58% | 10% | 21% | 1.50E-18g | 82 | 3.6 (2-4) | 65 | 0.85 |
|
| |||||||||||
| UAS:CA-Rac1el320 | 112 | 1.4 (0-3) | 93% | 0% | 0% | 7% | 2.25E-30g | 58 | 1.8 (1-3) | 55 | 0.18 |
| UAS:CA-Cdc42el322 | 138 | 3.1 (2-4) | 44% | 27% | 0% | 29% | 7.43E-22g | 64 | 3.2 (3-4) | 50 | 0.02 |
UAS transgenes were doubly transgenic with nkx2.3:Gal4VP16. The number (n) of analyzed embryos of each genotype is indicated for pouches and CB cartilages, with n for Alcama localization representing total number of cells analyzed. For pouches and CBs, the average number of identifiable separate elements is listed with the range of numbers in parentheses. For the scoring of Alcama localization (expressed as an average), strong membrane signal with either no or weak cytoplasmic signal was counted as 0, whereas strong cytoplasmic signal was counted as 1. p-values for differences in pouch number were calculated for the following comparisons:
wild type,
wnt11r,
fzd8a-MO (200uM),
wnt4a,
wnt11r;wnt4a,
and nkx2.3:Gal4VP16,
and for the difference in pouch morphology versus wild type.