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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Res Microbiol. 2012 Oct 24;163(0):674–684. doi: 10.1016/j.resmic.2012.10.013

Fig. 3. Biofilm formation and the Pi limitation effect require the UPP but not other polysaccharides.

Fig. 3

(A) CV-stained coverslip biofilms (48 h) of A. tumefaciens polysaccharide mutant derivatives grown in ATGN (high Pi, 79 mM) and Pi-limiting medium (low Pi, 50 μM). (B) Measurement of A600/OD600 values for acetic-acid-solubilized CV-stained 72-h biofilms under high and low Pi. Values are averages of triplicate assays and error bars are standard deviation. (C) Lectin labeling of A. tumefaciens C58 in short-term binding assays on PVC coverslip inoculated from planktonic cultures grown in ATGN and Pi-limiting medium. Cells were mixed with fl-DBA just prior to inoculation and, after 1 h, viewed at 100X magnification on a Nikon E800 epifluorescence microscope (excitation, 460-500 nm; emission, 510-560 nm) with an overlay of phase contrast and fluorescence images.