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a,b, Anti-HA immunoblot (a) and LC-MS analysis (b) of immortalized human astrocytes (passage 4) infected with retroviruses encoding HA-tagged versions of the indicated IDH1 variants. c,d, In vitro proliferation under standard culture conditions (c) or in soft agar (d)(passage 23). Bars in (d) = 0.5 mm. e, Number of macroscopic soft agar colonies in (d) (*P<0.01, **P<0.005). Error bars, s.d.; n=3.
