Skip to main content
. Author manuscript; available in PMC: 2013 May 17.
Published in final edited form as: Nature. 2012 Feb 15;483(7390):484–488. doi: 10.1038/nature10898

Fig 4. Decreased HIF activity contributes to transformation by mutant IDH.

Fig 4

a,c, Soft agar colony formation by human astrocytes after stable infection with lentiviruses encoding the indicated HIF1α shRNAs or scrambled (Scr) shRNA (a) or lentiviruses encoding EglN1, EglN1 (P317R), or venus fluorescent protein (c). Bars = 0.5 mm.

b,d, Number of macroscopic soft agar colonies in (a) and (c), respectively. (*P<0.05, **P<0.005). Error bars, s.d. n=3.

e,f, Proliferation (e) and immunoblot analysis (f) of human astrocytes expressing wild-type or R132H IDH1 and an shRNA against EglN1 (10578) (or scrambled control).

g,h, Proliferation of human astrocytes expressing IDH1 R132H and shRNAs against HIF1α (0819), EglN1 (10578), or both. Scr= scrambled shRNA.