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Competition of nonbiotinylated AF4 peptides with biotin–AF4 27-mer for binding to the AF9-FLAG protein. Nonbiotinylated AF4 peptides of varying lengths were tested for inhibition of binding between biotin-AF4 27-mer peptide and AF9-FLAG. The unlabeled peptides in the order of potency were AF4 27-mer (●), 12-mer (■), and 10-mer (▲). The 8-mer (♦) and inactive 12-mer (□) showed no inhibition of binding. Values=mean±SEM; n=3.
