Table 1.

AF4 Peptide Sequences

Peptide	Sequencea
Biotin-AF4 27-merb	Biotin-LSPLRDTPPPQSLMVKITLDLLSRIPQ-OH
AF4 27-mer	NH2-LSPLRDTPPPQSLMVKITLDLLSRIPQ-OH
8-merc	NH2-WVKIDLDL-OH
10-mer	NH2-LWVKIDLDLL-OH
12-mer	NH2-LWVKIDLDLLSR-OH
Inactive 12-merd	NH2-LWEKSDLDLLSR-OH

^aResidues key to AF9 binding are in bold type.

^bThe biotinylated and nonbiotinylated AF4 27-mer peptides are derived from residues 747–774 of AF4.

^cThe 8, 10, and 12-mer peptides are derived from the homolog FMR2, which is closely related to AF4 and shares the AF9 interaction domain. We chose to use these peptides to replicate the results found by Srinivasan et al.¹³

^dUnderline indicates substituted amino acids.