Table 1. Chromosomal location of the six genes (newly determined by in situ hybridization), the sequenced fragment length and primers used in the amplification and sequencing.
| Gene symbola | Chromosome location | Length (bp) | Primers |
|---|---|---|---|
| Pif1A (CG42599) | 79Db | 1820 | 5′-AATGTATCACAAGGAGAACG-3′c,d,e |
| 5′-CTCCTGGTAGTACTGCAGAT-3′c,d,e | |||
| 5′-GTCCAACTATGGTTGATGCC-3′d | |||
| 5′-ACGACTTTACAGCTAGCTGG-3′d | |||
| Abi (CG9749) | 81Ab | 1566 | 5′-CCTTGTCCGCATAGTAGTCA-3′c,d |
| 5′-GTGAGTAGTAATTAGGTTCG-3′c,d | |||
| 5′-ACTCACGTGGCATACTGCTC-3′d | |||
| Sqd (CG16901) | 85Db | 1436 | 5′-AACTAACCTTGTTCCTCTCC-3′c,e |
| 5′-TTACACACGCTTCGTCAGTT-3′c,d,e | |||
| 5′-CATTATAGTAGCCACCAGGA-3′d | |||
| 5′-TCTTGGTGGTTGTTGCTGTG-3′d | |||
| Yrt (CG9764) | 86E | 911 | 5′-CTGGACATCATCGAGAAGGA-3′c,d,e |
| 5′-ACATTGGCCAGCTTCACTTG-3′c,e | |||
| 5′-AACTGGAGTCTGGCTGGCGT-3′d | |||
| 5′-TACGCCGGATCATCGTTGCT-3′d | |||
| Atpα (CG5670) | 87C | 1490 | 5′-TCATAAGATCTCTCCTGAGG-3′c,d,e |
| 5′-GCAATATCCTCAACGGTCTC-3′c,d,e | |||
| 5′-CATCATTGAGGCGCGCAGCT-3′d | |||
| 5′-CCAGGGCTAGTTCTATCGTA-3′d | |||
| Fmr1 (CG6203) | 98Ab | 1984 | 5′-ACAGCCAAGTCGTTCTACCA-3′c,d,e |
| 5′-CCATTCACCAGACCTTCCTT-3′c,d,e | |||
| 5′-GGCAAGGTAATCGGCAAGAA-3′d |
Abbreviations: Abi, Abelson interacting protein; Atpα; Na pump α-subunit; Fmr1; Fragile X mental retardation; Pif1A, PFTAIRE-interacting factor 1A; Sqd, Squid; Yrt; Yurt.
a
Gene symbol of the homologous gene in D. melanogaster and annotation symbol in brackets.
b
Mislocated genes in Laayouni et al., 2007.
c
Primers used in the amplifications.
d
Primers used for sequencing.
e
Primers previously designed (Laayouni et al., 2007).