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. Author manuscript; available in PMC: 2014 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2012 Nov 20;366(1):31–37. doi: 10.1016/j.mce.2012.11.008

Figure 1. Inhibition of PLD-mediated signal production with 1-butanol inhibits AngII-induced PKD activation in primary cultures of bovine adrenal glomerulosa cells.

Figure 1

Primary cultures of bovine adrenal glomerulosa cells were incubated for 30 minutes with or without 10 nM AngII in the presence or absence of 0.3% 1-butanol. Cells were harvested from duplicate wells and analyzed by western blotting for PKD phosphorylation at serine 910. Panel A shows a representative immunoblot, whereas Panel B illustrates the combined results of 3 separate experiments performed in duplicate. Immunoreactivity of pSer910 was normalized using actin and then expressed relative to the normalized control value. Panel C demonstrates the inhibition by 1-butanol of AngII-elicited aldosterone secretion in the collected supernatants. *p<0.05, **p<0.01, ***p<0.001 versus the control, †p<0.05, ††p<0.01 versus AngII alone by ANOVA followed by a Student-Newmann-Keuls post-hoc test. (Note that the lower band seen in some lanes of the representative western blot was not consistently observed and is likely non-specific.)