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. Author manuscript; available in PMC: 2014 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2012 Nov 20;366(1):31–37. doi: 10.1016/j.mce.2012.11.008

Figure 5. FIPI inhibits AngII-induced, but not 22(R)hydroxycholesterol-mediated aldosterone production in HAC15 human adrenocortical carcinoma cells.

Figure 5

HAC15 cells incubated for 20–24 hours in low-serum medium were treated with low-serum medium in the presence of 750 nM FIPI or the vehicle control (0.1% DMSO) with or without 10 nM AngII for 18 hours. In other wells 22(R)-Hydroxycholesterol (2 μM) was added, and experimental medium was collected 6 hours later (for a total incubation time of 24 hours) and stored frozen until assayed. Aldosterone levels were then measured using a solid-phase radioimmunoassay and expressed as the –fold relative to the control (basal) levels. Values represent the means ± SEM of 3 separate experiments performed in triplicate; *p<0.05 versus the control; †p<0.05 versus AngII alone.