Table 2. Characterization of modifier alleles.
| EP# | Gene | Insertion Site (position) | Mutation | C96-domR | N Wings Scored | C96-MamH | N Wings Scored |
|---|---|---|---|---|---|---|---|
| 425 | tara | Intron (12075314) | LOF | S (5%) | 414 | S+ | 92 |
| 558 | pabp2 | Exon (4019484) | LOF | E (88%) | 312 | E | 100 |
| 573 | Lk6 | Intron (7585856) | LOF | E (68%) | 1023 | S | 206 |
| 593 | Tudor-SN | Upstream (264378) | GOF | E (74%) | 426 | ne | 288 |
| 939 | EcR | Intron (2007989) | LOF | E (88%) | 336 | E | 110 |
| 1000 | lola | Not Determined | LOF | E (91%) | 251 | E+ | 100 |
| 1037 | wdb | Intron (23402526) | GOF | S (20%) | 368 | S+ | 98 |
| 1202 | atg1 | Exon (12798085) | LOF | S (0%) | 338 | S+ | 90 |
| 1538 | lola | Intron (6421948) | LOF | E (91%) | 330 | E+ | 86 |
| 1561 | emc | Upstream (749363) | GOF | E (85%) | 240 | S+ | 130 |
| 1630 | lilli | Intron (2900668) | GOF | E (81%) | 378 | E+ | 194 |
| 1646 | pum | Intron (4983814) | LOF | E (70%) | 250 | E+ | 130 |
The percentage of wing nicking is shown for crosses of C96-domR to EP modifiers in column labeled C96-domR. All wing nicking differences were highly significant (P < 0.001, chi-square test relative to w1118 control; 57%, Table 1). All EP tests included w1118 controls, and wing nicking percentages were normalized to 57% control value between experiments. EP 1000 was determined to be an allele of lola through a complementation test. The C96-MamH genotype produces 100% wing nicking when outcrossed to w1118 control. Among this control class we determined the percent of wings with weak, moderate, and strong effects including extent of nicks and blade loss. We then compared the distribution of severity in the EP cross progeny to determine if there was suppression (S), strong suppression (S+), enhancement (E), strong enhancement (E+) or no effect (ne); these data are presented in column labeled C96-MamH. GOF, gain of function; LOF, loss of function.