FIG. 3.

Binding of selected phage to heterologous OppA proteins. The ability of phage selected for binding to a specific OppA protein to bind to other OppA proteins was tested. Proteins were used to coat wells of a 96-well plate and were incubated in the presence of phage expressing a specific heptamer. Phage titers of 10¹⁰ PFU for A1-9, A1-10, and A1-64 and of 10¹¹ PFU for A2-8, A3-1, A3-3, and NB-21 (NB-) were used. After the wells were washed, bound phage were quantitated with an HRP-conjugated anti-M13 antibody. The OD₄₀₅ was determined after incubation with an HRP-labeled substrate. Error bars represent standard errors of the means from three experiments performed in duplicate.