(A) EdGLU was measured as 750 μM PDC was perfused
(gray line) through the probe. Ed (black solid circles) values
reached the minimum value within 5 min (p-value <
0.001 compared to aCSF). With 2 μM 13C5-GLU perfused through the probe, the addition of PDC to the perfusate
increased [13C5-GLU measured] (right axis, black
open circles). (B) [12C-GLU measured] (right axis, black
open circles), CappGLU with constant Ed assumed (EdGLU measured prior to PDC, gray solid circles), and Capp (black
solid circles) were measured as PDC was perfused. CappGLU was
on average 190% higher compared to Capp with a constant EdGLU assumed
(p-value < 0.001). (C) % baseline of GLU for calibrated
(black solid circles) vs conventional (black open circles) microdialysis
is shown. The calibrated measurements were 200% larger compared to
the conventional microdialysis (p-value < 0.001).
Paired t tests were used for individual fractions
to test significance as well. Error bars show SEM (n = 4). ** indicates p-value < 0.01, and * indicates p-value < 0.05.