Table 1.
Number of protein identifications using the whole gel (WG) procedure vs in gel digestion (IGD)
| Human HCT116 cell lysate | Mouse tumor tissue lysate | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Gel Band |
1 |
2 |
3 |
4 |
5 |
1 |
2 |
3 |
4 |
5 |
6 |
| Total ID IGD |
178 |
252 |
300 |
301 |
93 |
86 |
113 |
70 |
100 |
28 |
23 |
| Total ID WG |
178 |
257 |
306 |
315 |
92 |
56 |
115 |
78 |
114 |
52 |
62 |
| Total ID |
192 |
262 |
314 |
324 |
95 |
86 |
121 |
81 |
114 |
52 |
62 |
| Overlap ID |
164 |
247 |
292 |
292 |
90 |
56 |
107 |
67 |
100 |
28 |
23 |
| Only IGD |
14 |
5 |
8 |
9 |
3 |
30 |
6 |
3 |
0 |
0 |
0 |
| Only WG |
14 |
10 |
14 |
23 |
2 |
0 |
8 |
11 |
14 |
24 |
39 |
| Overlap* |
85% |
94% |
93% |
90% |
95% |
65% |
88% |
83% |
88% |
54% |
37% |
| Median Mr (kDa) | 176 | 83 | 44 | 23 | 12 | 124 | 69 | 38 | 29.5 | 19 | 15 |
*Overlap =(Overlap ID/total ID)*100%.