Figure 4.

Intracellular cytokine staining of splenocytes after AdV boosting immunization. (A) The CD8⁺/IFN-γ⁺ staining. (B) The CD4⁺/IL-4⁺ staining results. (C and D) The double-positive percentage of CD8⁺/IFN-γ⁺ and CD4⁺/IL-4⁺.

Notes: The data were represented as mean ± standard deviation of three independent experiments. The double-positive percentage of CD4⁺/IL-4⁺ and CD8⁺/IFN-γ⁺ of the m-PEI/DNA group was significantly higher than that of the naked DNA group and Mock, *P < 0.05; ***P < 0.005.

Abbreviations: AdV, adenovirus; IFN, interferon; PE, polyethyleneimine; DNA, deoxyribonucleic acid; m-PEI/DNA, group primed with a complex of mannosylated polyethyleneimine and DNA plasmid and boosted with AdV; Mock, group primed with empty vector pVAX1 and boosted with AdV; FITC, fluorescein isothiocyanate; IL, interleukin.