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. Author manuscript; available in PMC: 2013 May 17.
Published in final edited form as: Cell Stem Cell. 2010 Sep 30;7(5):618–630. doi: 10.1016/j.stem.2010.08.012

Figure 4. Trilineage differentiation of RiPS cells.

Figure 4

(A) Yield and typology of blood-lineage colonies produced by directed differentiation of embryoid bodies in methylcellulose assays with RiPS clones derived from BJ, CF, D551 and MCR5 fibroblasts, and a human ES (H1) control. (B) Immunostaining showing expression of the lineage markers Tuj1 (neuronal, ectodermal), and alpha-fetoprotein (epithelial, endodermal) in RiPS clones from 3 independent RiPS derivations subjected to directed differentiation. Hematoxylin and eosin staining of BJ-, CF- and dH1F-RiPS-derived teratomas showing histological overview, ectoderm (pigmented epithelia (BJ and CF), neural rosettes (dH1F)), mesoderm (cartilage, all), and endoderm (gut-like endothelium, all). For blood formation and methylcellulose assays, n=3 for each clone. See also Figure S5.