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Dose-dependent copper loading of J774 cells. J774 macrophages were incubated with the indicated concentrations of CuSO4 for 20 h. Cells were extensively washed and digested with concentrated nitric acid overnight before copper analysis by inductively coupled plasma mass spectrometry (ICP-MS). Values were normalized to the cell protein content of the samples and shown are the means ± SD (n = 5) obtained from two individual experiments.
