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. 2013 May 17;8(5):e63914. doi: 10.1371/journal.pone.0063914

Figure 1. Purification and identification of cN-II in extracts of N yeast strain.

Figure 1

A) Immunoblot analysis of Y and N strain crude extracts. Lane 1∶10 µg of protein of Y strain. Lane 2∶10 µg of protein of N strain. B) Elution profile of cN-II from N strain extract from IMAC resin. cN-II phosphotransferase activity is reported as DPM after 30 min of assay as described in Experimental Procedures (-•-). Dashed line (---) represents the imidazole gradient. Chromatography conditions were as described in Experimental Procedures. Y strain extracts, treated in the same way, showed no phosphotransferase activity in the same fractions (data not shown). C) SDS-PAGE of purified (as described in B) N and Y strain extracts. Lanes are as described in panel A. In this case 5 µg of proteins were loaded (lane 1 and 2).