Table 3. Pools of nucleosides and nucleotides in yeast cell extracts.
Na | Ya | statistics | |
UTP | 41±16 | 136±49 | p = 0.033 |
GTP | 41±14 | 123±45 | p = 0.04 |
ATP | 374±46 | 1175±300 | p = 0.01 |
CTP | 52±20 | 214±87 | p = 0.03 |
UDP | 66±19 | 93±48 | NS |
GDP | 53±10 | 39±10 | NS |
ADP | 441±66 | 304±133 | NS |
CDP | 53±13 | 84±20 | NS |
UMP | 115±28 | nd | |
GMP | 77±5 | nd | |
AMP | 384±109 | 86±42 | p = 0.012 |
IMP | 62±10 | nd | |
Inosine | 78±9 | 362±17 | p<0.0001 |
Ipoxanthine | 176±47 | 399±12 | p<0.0001 |
UDPG | 343±39 | 571±178 | NS |
NAD | 1374±313 | 1494±374 | NS |
NADP | 67±27 | 40±10 | NS |
AXP+IMP | 1337±212 | 1590±524 | NS |
GXP | 172±28 | 186±52 | NS |
AMP/ATP | 1.1±0.14 | 0.0955±0.0096 | p = 0.0098 |
EC | 0.537±0.081 | 0.83±0.036 | p = 0.0047 |
Shadowed are the compounds in which a significant variation was measured between N and Y strain. Values are the mean± SD of at least three independent samples, and are expressed in arbitrary units. Each value is calculated dividing the peak area in the electropherogram by the value of protein concentration of the pellet of the same sample (see Experimental Procedures).
nd = not detectable; NS = not significant; UDPG = UDP-glucose; AXP = ATP+ADP+AMP; GXP = GTP+GDP+GMP; EC = energy charge.
N represents the strain expressing cN-II whereas Y the control strain.