Figure 1. Effect of extracellular alkalinization on pHi in isolated endothelial cells from rat aorta.
Cells were loaded with SNARF-1 (10 µM) and analyzed by flow cytometry. NaOH was used to increase pHo from 7.4 to 8.0 and from 7.4 to 8.5; Hanks solution pH 7.4 served as control. Fluorescence intensity was measured before the NaOH stimulus (F0) and at different time points (t = 1, 3, 9 and 15 min) after this stimulus (Ft). Results are reported as ΔF = Ft - F0. All values are means ± SEM (n = 7). Two-way ANOVA, Bonferroni's post-test, * p<0.05 versus control.