Skip to main content
. 2013 May 17;8(5):e64051. doi: 10.1371/journal.pone.0064051

Figure 1. Metformin inhibits the growth of pediatric glioma cell lines in response to 2DG.

Figure 1

Pediatric glioma cells were cultured in 25 mM glucose in the presence of 2DG (10 mM), metformin (8 mM) either alone or in combination. Cell viability was assessed by WST-1 cleavage after 24, 48 and 72 hours (A(i)–D(i)), whilst effects on cell proliferation were measured by fluorescent quantification of cell density (A(ii)–D(ii)). For WST-1 assays results are expressed as mean percentages relative to mock treated cells. Error bars represent the standard error of the mean from three independent experiments, repeated in triplicate. Statistical significance was determined using Kruskal-Wallis ANOVA followed by Bonferroni corrected Mann-Whitney U tests: *P≤0.05, **P≤0.01, 2DG and metformin treated cells vs. single agents.