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. 2013 May 17;8(5):e64453. doi: 10.1371/journal.pone.0064453

Figure 2. Activation of the kinin B1 receptor in the absence of kinin B2 receptor inhibits melanoma cell migration in vitro.

Figure 2

Wound healing assays were performed to evaluate the role of the B1 receptor in melanoma cell migration, (A) representative image and quantification of healing (B) and single cell migration (C) of 4 independent experiments in triplicate. (D) Cell viability was accessed using the MTT assay 24 and 48 h after DABK or DLBK stimulation; (E) E-cadherin mRNA expression levels were evaluated by RT-qPCR 24 h after B1 receptor agonist stimulation. Introduction of kinin B2 receptor in B2 receptor-free cells abrogates DABK-mediated migration inhibition (F–H). (All results are from 2–3 independent experiments performed in triplicate, unless otherwise stated). DABK: desArg9-bradykinin; DLBK: desArg9-[Leu8]-bradykinin. Data are expressed as the mean ± SEM; * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001. The scale bars represent 200 μm.