Fig. 4.

(A) Conformational rearrangement of the MVM capsid at moderate temperatures. Open circles, wild type; inverted triangles, mutant S550A; and upright triangles, mutant V40A. (B) Dissociation of the MVM capsid at high temperatures. Open circles, wild type; inverted triangles, mutant H72A; upright triangles, mutant N149A at neutral pH; and diamonds, wild type at pH 2.7. In A and B, the curves were obtained in tryptophan emission fluorescence experiments and have been fitted to sigmoidal cooperative transitions and offset for clarity. The negative slope of the baselines corresponds to thermal quenching. a.u., arbitrary units.