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Energy diagram for the enzymatic and nonenzymatic isomerization of CHN to flavanone. The energy diagram in the enzymatic reaction considers only active site B conformation. Active site A conformation is not reactive, represents about half of the enzyme concentration, and thus can be ignored in the overall reaction coordinate. Numbers in parentheses are free energy contents (kilocalories per mole) relative to II in water. Numbers with * are from experimental data (refs. 1 and 2).
