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(A) Semiquantitative immunoblotting at 3 or 6 h after treatment with 10 µM HHE. The expression of total cellular β-catenin protein was increased at 3 h and continued to rise at 6 h in HHE-treated HK-2 cells. (B) Similarly, nuclear β-catenin was increased at 3 h and maintained at 6 h after HHE treatment. *P<0.05 compared to the control.
