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|
Kd, μM
|
||
|---|---|---|
| FixL | –ADP | +ADP |
| Wild type | 56 ± 8 | 218 ± 27 |
| N403Q | 42 ± 1 | 40 ± 7 |
| N403E | 47 ± 2 | 45 ± 1 |
| N403C | 79 ± 2 | 73 ± 2 |
| H285V | 69 ± 1 | 173 ± 21 |
Kd values of FixLs were determined in the presence of FixJ from the OECs at 25°C. The concentrations of FixL and FixJ in the kinase buffer were 5 and 10 μM, respectively. MnCl2 and ADP were added at final concentrations of 0.2 mM.
