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. 2004 Feb 19;101(9):2747–2751. doi: 10.1073/pnas.0307343101

Fig. 1.

Fig. 1.

(A) Fold overexpression of trp operon genes from microarray experiments. The order of genes in the aromatic amino acid supraoperon is depicted. Promoters are indicated by hooked arrows. Numbers below genes indicate level of expression in the PNPase- strain relative to wild type. Values are averages of four determinations, two measurements each from two independent RNA isolations. (B) Northern blot analysis of trp operon mRNA in wild-type and pnpA strains. Total RNA was separated on a formaldehydeagarose gel and probed with a uniformly labeled trpA antisense RNA. Migration of 23S and 16S ribosomal RNAs is indicated. Strains were grown in the presence (+) or absence (-) of tryptophan. (C) Northern blot analysis of trp operon mRNA in the pnpA mutant strain, carrying no plasmid (lane 1), vector plasmid + mtrB gene (lane 2), and vector plasmid alone (lane 3). The probe for both Northern blots shown was an antisense trpA probe.