Fig. 3.

Analysis of 114-nt trp leader RNA in B. subtilis protein extracts. (A) Low-resolution polyacrylamide gels. Size marker DNA fragments were in lane M. Control (lane C) contained substrate RNA that was not incubated in the extract. Substrate RNA (migration indicated by arrows) was incubated in the extract without (lanes 1–3) or with (lanes 4–6) addition of 1.25 mM tryptophan. Additions were none (lanes 1 and 4), 1 mM Mn²⁺ (lanes 2 and 5), or 1 mM Mn²⁺ + 1 mM NaH₂PO₄ (lanes 3 and 6). Results using wild-type cell extract (Left) and pnpA mutant cell extract (Right) are shown. (B) High-resolution polyacrylamide gel. Samples loaded in lanes 1–6 are the same as those in lanes 1–6 in A Left. Migration of substrate RNA is indicated by the arrow at the left. A DNA sequencing ladder is at the right, with sizes (in nt) indicated.