Table 1. Cell lines used in the study.
Name of cell line or cell line stock | Number of cell line | Primary tissue | Real identity | Source (Institution) | Address | Year of acquisition | Origin and history** |
UROtsa-1 | – | Immortalized normal urothelium | UROtsa | J.R. Masters (University College London) | London, UK | 2011 | [3] |
UROtsa-2 | – | Immortalized normal urothelium | (UROtsa)*** | S.H. Garrett (University of North Dakota) | Grand Forks, ND | 2005 | [3], [4] |
UROtsa-3 | – | Transitional cell carcinoma of the urinary bladder** | T24 | M. Styblo (University of North Carolina) | Chapel Hill, NC | 2008 | [3], [76], [77] |
UROtsa-4 | – | Immortalized normal urothelium | UROtsa | A. Fabarius (University of Heidelberg) | Mannheim, Germany | 2011 | [3], [5], [78] |
UROtsa/F35 | – | Transduced UROtsa-3** | T24 | M. Styblo (University of North Carolina) | Chapel Hill, NC | 2011 | [3], [25], [76], [77] |
T24 | ACC 376 | Transitional cell carcinoma of the urinary bladder | – | DSMZ | Braunschweig, Germany | 2011 | – |
HeLa S3 | ACC 161 | Cervix carcinoma | – | DSMZ | Braunschweig, Germany | 2011 | – |
HepG2 | HB-8065 | Hepatocellular carcinoma | – | ATCC | Manassas, VA | 2003 | – |
RT4 | HTB-2 | Well-differentiated transitional papillary tumor of the urinary bladder | – | ATCC | Manassas, VA | 2006 | – |
BEAS-2B | CRL-9609 | Immortalized normal bronchial epithelium | – | ATCC | Manassas, VA | 2011 | – |
HUEPC | 536×090206* 459×250403* | Primary urothelium | – | Provitro GmbH | Berlin, Germany | 2012 | – |
DSMZ: German Collection of Microorganisms and Cell Cultures, ATCC: American Type Culture Collection.
Lot numbers.
All UROtsa stocks originated in the lab of I.M. Leigh and J.R. Masters [3] and were then handed from lab to lab, as described in the cited literature. We created the numbering to distinguish the different stocks. UROtsa-3 was cross-contaminated with T24 at some time point and UROtsa/F35 was derived from UROtsa-3. Therefore, both are not authentic UROtsa cell line stocks but T24 cells. Based on STR profiling only UROtsa-1 and UROtsa-4 are authentic.
UROtsa-2 was not tested by STR profiling but showed (in contrast to T24) to have normal chromosomal numbers like authentic UROtsa.