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. 2013 May 17;8(5):e64139. doi: 10.1371/journal.pone.0064139

Table 1. Cell lines used in the study.

Name of cell line or cell line stock Number of cell line Primary tissue Real identity Source (Institution) Address Year of acquisition Origin and history**
UROtsa-1 Immortalized normal urothelium UROtsa J.R. Masters (University College London) London, UK 2011 [3]
UROtsa-2 Immortalized normal urothelium (UROtsa)*** S.H. Garrett (University of North Dakota) Grand Forks, ND 2005 [3], [4]
UROtsa-3 Transitional cell carcinoma of the urinary bladder** T24 M. Styblo (University of North Carolina) Chapel Hill, NC 2008 [3], [76], [77]
UROtsa-4 Immortalized normal urothelium UROtsa A. Fabarius (University of Heidelberg) Mannheim, Germany 2011 [3], [5], [78]
UROtsa/F35 Transduced UROtsa-3** T24 M. Styblo (University of North Carolina) Chapel Hill, NC 2011 [3], [25], [76], [77]
T24 ACC 376 Transitional cell carcinoma of the urinary bladder DSMZ Braunschweig, Germany 2011
HeLa S3 ACC 161 Cervix carcinoma DSMZ Braunschweig, Germany 2011
HepG2 HB-8065 Hepatocellular carcinoma ATCC Manassas, VA 2003
RT4 HTB-2 Well-differentiated transitional papillary tumor of the urinary bladder ATCC Manassas, VA 2006
BEAS-2B CRL-9609 Immortalized normal bronchial epithelium ATCC Manassas, VA 2011
HUEPC 536×090206* 459×250403* Primary urothelium Provitro GmbH Berlin, Germany 2012

DSMZ: German Collection of Microorganisms and Cell Cultures, ATCC: American Type Culture Collection.

*

Lot numbers.

**

All UROtsa stocks originated in the lab of I.M. Leigh and J.R. Masters [3] and were then handed from lab to lab, as described in the cited literature. We created the numbering to distinguish the different stocks. UROtsa-3 was cross-contaminated with T24 at some time point and UROtsa/F35 was derived from UROtsa-3. Therefore, both are not authentic UROtsa cell line stocks but T24 cells. Based on STR profiling only UROtsa-1 and UROtsa-4 are authentic.

***

UROtsa-2 was not tested by STR profiling but showed (in contrast to T24) to have normal chromosomal numbers like authentic UROtsa.