Fig. 3.

Cleavage rates for Int and charge reversal substitution mutants on full-att and half-att site suicide substrates. The amount of covalent complex formed by Int (diamonds), R169D (triangles), and E153R (squares) on a full-att (A) or a half-att (B) suicide substrate is shown as a function of time. The reactions, containing 20 nM DNA and 50 nM protein, were carried out and analyzed as described in Fig. 2.