Figure 4. Verification of hematopoietic potential of Lin⁻Sca-1⁺CD45⁻c-Kit⁺ or Lin⁻Sca-1⁺CD45⁺c-Kit⁺ (positive control) cells by single cell-derived colony in-vitro assay.

(A) Quantitative analysis of colony formation efficacy. (B) Example of a colony formed by Lin⁻Sca-1⁺CD45⁺cKit⁺ cells (contrast-phase microscopy and Wright’s staining) containing relatively homogenous progeny. *** - p<0.001 versus Lin⁻Sca-1⁺CD45⁺c-Kit⁺ cells.